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2.6 Metabolic pathway enrichment analysis The HMDB IDs of known metabolites were retrieved from The Human Metabolome Database ( and enrichment analysis of the metabolic pathways associated with these metabolites was conducted using MetaboAnalyst 5.0 ( The pathway libraries selected for this analysis were the Small Molecule Pathway Database (SMPDB) and the Kyoto Encyclopedia of Genes and Genomes (KEGG)

Even though the preservative inhibits bacterial growth, it doesnt guarantee sterility if grossly contaminated, so proper handling is essential (wiping the rubber stopper with alcohol each time, using new sterile needles, etc.)

Astragaloside IV derivative (LS-102) alleviated myocardial ischemia reperfusion injury by inhibiting Drp1(Ser616) phosphorylation-mediated mitochondrial fission