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Although both ENDO3 and ENDO12 contain an azo functional group, their inhibitory effect is specific, as they effectively inhibited eTLR activation in the SEAP colorimetric assay, in selective fluorescence-based TR-FRET assays and in immunological assays using primary cells with cytokine production as the readout

Establishing a long-term prospective registry study has been suggested in a recent Endocrine Society Scientific Statement on this topic (Pope et al., 2014) and this could prove very valuable in determining the long-term safety of PEDs

Evidence is predominantly preclinical, with heterogeneous methods and sparse PD-specific randomized trials