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Effects of intensive glucose control on microvascular outcomes in patients with type 2 diabetes: a meta-analysis of individual participant data from randomised controlled trials

West Bloomfield, MI: Medical Alternatives Press

Cells were then harvested by centrifugation at 1,000 g for 3 min at room temperature and resuspended in 100 mM MES/Tris buffer, pH 6, to an OD 600 of 7.5