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This creates a devastating feedback loop: the lack of glutathione allows oxidative stress to run rampant, which further impairs the methylation cycle, which in turn prevents the production of more glutathione

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ATP-dependent RNA helicase DHX8 (DHX8) and hypoxanthine-guanine phosphoribosyltransferase (HPRT) were used as endogenous controls for standardization of relative mRNA expression in phLF and phBEC, respectively

Negreira et al., 2023), and mixed-genotype infections are frequently observed even within the same host tissue ( Leishmania is driven by several mechanisms, including mosaic aneuploidya common feature that enables rapid adaptation under stress, with non-random, strain-specific patterns indicating selective pressure (Sterkers et al., 2014