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The dendritic cells that were activated were incubated with MAP (antigen) for 3 hours at a 37C incubator with 5% CO2

Legal challenges to these deadlines were filed, and the regulatory landscape continues to evolve

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Briefly, 3T3-L1 cells at 50% confluence were transfected with 10 g plasmids (pcDNA3.1 or pcDNA3.1-SIRT3) using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Grand Island, NY, USA)