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Protein purifications Cdc48, Npl4, and their variants were expressed and purified as previously described 17

However, only pimitespib, an oral inhibitor developed by Taiho Pharmaceutical, was approved in Japan in 2022 for gastrointestinal stromal tumors, and it is currently the focus of a phase I study for the treatment of solid tumors in the EU and USA [7, 8]