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This preparation was used in in vitro assays on human fecal slurry while monitoring the levels of TMA released over time, and it was also tested in vivo in both Mus musculus C57BL/6 (FMO3 +/+ ) strain WT and C57BL/6 -Fmo3em1Smoc (KO) mouse models to measure the trimethylamine N-oxide (TMAO) and TMA levels in the blood and urine, along with gut microbiota analysis in feces via next-generation sequencing (NGS)

It is believed to be inherited as a polygenic threshold trait with a high rate of heritability in some breeds and is considered to be hereditary in the CKCS