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Pharmacology of anabolic steroids

To estimate the cytosolic pH, cells were incubated with 2.5 M of BCECF AM for 30 min in 37 C and then analyzed in Live cell imaging solution with the Multimode plate reader EnSpire (PerkinElmer), and the ratio was calculated from dualexcitation with 1 = 490 nm and 2 = 440 nm and fixed emission at 535 nm