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Thus, after MH or SP treatment, gene expression in the ROT group was restored such that it approximated to the characteristics of the NC group, suggesting a specific effect of MH or SP in remodeling the expression pattern of PD-associated genes

For H 2 O 2 treatment, 100 M H 2 O 2 was added to RPMI 1640 growth medium (#1003461059, Sigma Aldrich, United Kingdom), without FBS and phenol red, supplemented with 15 mM HEPES (#1003098482, Sigma Aldrich, USA), 27.9 mM sodium bicarbonate (#101920550, Sigma Aldrich, Japan), 0.2 mM sodium pyruvate (#1002136564, Sigma Aldrich, Japan), 1 U/mL penicillin, and 1 g/mL streptomycin

Inconsistency: Skipping days makes it harder for your body to maintain optimal levels

This enzyme converts nitrite to nitric oxide