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Of note, enzymatic GSH, GSSG, and GSH:GSSG measurements corresponded well with measures using a high-performance liquid chromatography system (Supplemental Figure 1)

The WD40 domains of COP1 b and COP1 d interact with DET1 in a manner similar to the COP1-DDD-Ube2e dimer (described below), while the RING dimers are in close proximity to Ube2e2 (Fig