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279 In Escherichia coli , an elevated ratio of NADH to NAD + inhibits pyruvate dehydrogenase (PDH) activity, impeding the formation of acetyl-CoA, as the conversion of pyruvate to acetyl-CoA relies on PDH

62,99,100 Together, these reports provide strong evidence that after an APAP overdose, in vivo and in isolated hepatocytes in culture, ferrous iron can be released from lysosomes and then taken up into mitochondria via the MCFU, where it contributes to MPTP opening and cell death (Fig