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This assay was also carried out in primary astrocytes to determine cell viability in non-tumor cells ( Supplementary Material )

Primers were identified and removed using cutadapt (v2.7) with a maximum error rate of 0.2, reads lacking both forward and reverse primers were discarded

AlphaPlex is a further development that allows quantification of up to three analytes by using different acceptor beads that emit at distinct wavelengths