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Briefly, 10 6 BEAS-2B cells or HBEC were recovered using EDTA-free trypsin, counted and incubated in serum-free DMEM with 0.2 M Calcein-AM at 37 C for 7 min

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In PK deficiency, 2,3-BPG is increased as a result of the metabolic block at the PK step and of retrograde accumulation of products of glycolysis