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NV thanks FCT by IF position, Fundao Manuel Antnio da Mota (FMAM, Portugal) and Pfizer Portugal for research group support

2.13 Co-immunoprecipitation assay Co-immunoprecipitation (Co-IP) assay was performed as described previously ( 2.14 Surface plasmon resonance (SPR) assay To evaluate the direct binding affinity between Hyp and STAT3 protein, a SPR assay was performed using a Biacore 8K system (GE Healthcare)

Jain K, Kesharwani P, Gupta U, Jain NK

The degradation of proteins primarily occurs through the UPS and autophagosomal lysosomal system (ALS), with the UPS responsible for the degradation of approximately 80% of proteins, particularly those that are short-lived, regulatory, damaged, or misfolded[1]