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After centrifugation, the homogenate was incubated with 200ml of KPBS pre-washed anti-HA magnetic beads on a rotator shaker for 5min at 4C

Moreover, A study by Hosseinzadeh et al

NMT nicotinamide N-methyltransferase Nicotinamide N-methyltransferase is a key enzyme located in the cytosolic milieu that catalyzes the transfer of methyl group from the co-factor S-(5-Adenosyl)-L-methionine (SAM) to substrates such as nicotinamide (NCA), pyridine, and related analogs, such as quinoline, isoquinoline, and the aliphatic amine 1,2,3,4 tetrahydroisoquinoline