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One day after seeding, the cells were treated with either 50 nM MitoQ, 100 nM MitoQ, 10 M retinoic acid or vehicle, and incubated 37C for 7 days

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(Green R, et al., Nature Reviews Disease Primers, 2017 PubMed: 28660890 ) Because B12 is water-soluble, excess amounts that the body cannot use are excreted in urine rather than stored