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The strategy of interrogating a single growth-hormone-axis pathway through a defined molecule parallels the contemporaneous lineage of growth-hormone secretagogues such as CJC-1295 with DAC, which pursued the complementary aim of prolonging endogenous GH release rather than isolating a fragment

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Absorbance corresponding to the amount of formazan dye produced by treated cells was normalized to that produced by control (untreated) cells to calculate % viable cells in the treated samples