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All of the detectable intermediates and products, including aldimine and pyruvate, were only found with the addition of Fe 3+ (Table 2 and Supplementary Fig

By exploiting improvements in techniques for the isolation of intact spinach chloroplasts that retain the chloroplast envelope and therefore stromal components (as well as the thylakoid membranes), the authors were able to locate appreciable GR activity and contents of glutathione in this organelle