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Reactions were performed with 0.001 mg/ml DNA substrate Poly(dI-dC), 1 M radiolabelled S-adenosyl-L-[methyl-3H] methionine (SAM) co-substrate, and recombinant human DNMT1 enzyme

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Enzymes that consume NAD + generate NAM as a product, and this can be recycled in the salvage pathway, providing the primary NAD + synthesis pathway in mammals 30