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Quantitative metabolite profiling typically comes after qualitative profiling because the understanding of how much is often as important as what is it, especially as the drug makes its way along the discovery and development path

For instance, the probiotic strain Lactococcus lactis MG1363-pMG36e-GLP-1 activates the Nrf2/GPX4 signaling pathway to inhibit ferroptosis, ameliorating motor deficits in PD mice (Yue et al., 2022)

In this chapter, we explain the widely used GST pull-down assay to identify the proteinprotein interaction between purified proteins

Deficiency of hepatocystin induces autophagy through an Mtor-dependent pathway