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The purified GST- jr PPO1-wt fusion protein was exchanged to a glutathione-free buffer containing 200 mM NaCl and 50 mM Tris-HCl at pH 7.8 and 1 mM dithiothreitol (DTT) using a Vivaspin ultrafiltration device (VWR, molecular weight cut-off 30 kDa)

Winter 2007;9(1):17-27

In nonadipocytes, the perilipin-related protein adipocyte differentiation-related protein/adipophilin replaces perilipin at the surface of neutral lipid storage droplets, although this protein is largely absent in mature adipocytes (13)

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