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After a 1-min habituation period, the fish were subjected to water flow resistance, and their U crit (critical swimming speed, represented as cm/s) was calculated using a formula based on maximum velocity and time to fatigue as described earlier 28

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Notably, alterations in glucose and lipid metabolism pathways, as well as changes in the overall metabolic microenvironment, significantly impact DC maturation, antigen presentation, and immunoregulatory functions