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Doses and schedules would be pre-registered, and safety monitoring would track copper status (serum copper, ceruloplasmin), injection-site reactions, and any systemic effects

Barcodes for each sample were introduced in a first PCR, the product of which were purified using Mag-Bind TotalPure NGS beads (Omega Bio-tek) and amplified in a second PCR introducing the standard Illumina adapters

The pattern of oxygen use was not seen as significant either in cold- or warm-acclimated fish (Robinson and Davison, 2008)

Translating ROS from a complex biological variable into a clinically actionable target will require a multi-pronged, precision-based strategy