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Notice its a caution-and-compensation argument, not a the effect wears off argument

a concept for neuron vulnerability in Parkinson's disease

10.1179/174329211X13002357050897 [DOI] [PMC free article] [PubMed] [Google Scholar] Shin I.-S., Shin N.-R., Jeon C.-M., Hong J.-M., Kwon O.-K., Kim J.-C., et al

In addition to culturing on SD/-Leu plates to select the transformants, a series of dilution of the transformed mixture was spread on SD/-Leu media to calculate the transformation efficiency and independent colonies