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RNA Isolation, cDNA Synthesis and Reverse Transcription Quantitative Real Time Polymerase Chain Reaction Analysis Cells were collected for RNA isolation by washing with PBS (Life technologies), scraping the cells into PBS followed by centrifugation at 12 000 g for 1 minute

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Additionally, vitamin B12 deficiency may negatively impact pregnancy and fetal development, underscoring the need for further research into its role in womens health (15)