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Statistical significance was evaluated using JMP v.16.1.0 software (SAS)

Substrate solution (200 l) was added to each well and incubated at RT for 15 min, and the reaction stopped by the addition of stop solution (100 l)

Human blood samples from healthy donors were collected and processed at the University of Rochester Medical Center following Institutional Review Board approval

Recent studies demonstrate that ferroptosis not only shapes innate and adaptive immune responses-including T and B lymphocytes, macrophages, neutrophils, and dendritic cells-but also remodels inflammatory microenvironments to either preserve tissue integrity or exacerbate damage