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To assess protein interactions between G3BP1 and SNARE complex components in MASLD/MASH pathogenesis, liver tissues from control and disease model mice were homogenized in ice-cold lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 1 protease inhibitor cocktail, Roche, 05892953001) and centrifuged at 12,000 g for 15 min at 4 C to collect supernatants

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Globally, an estimated 61.8 million individuals were living with ASD in 2021 (Santomauro et al., 2025)