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Cognitive difficulties: B12 plays a role in brain function, and its deficiency can lead to problems with memory, concentration, and cognitive skills

Reactions were terminated by heating at 98 C for 2 min to inactivate Proteinase K, followed by reduction with 10 mM dithiothreitol at 37 C for 30 min and alkylation with 40 mM iodoacetamide at 37 C for 45 min in the dark

The most likely exchanged amino acids by SLC1A5 are asparagine, threonine, or serine, and their transport, together with glutamine, allows the entry of 12 carbon atoms into the cell, which can then be oxidized in the TCA with ATP production in the mitochondria

Funding This work was supported by the Biotechnology and Biological Sciences Research Council (grant number BB/T0083690/1)