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A later study using isobaric tag for relative and absolute quantitation (iTRAQ) and one-dimensional gel electrophoresis-Fourier transform mass spectrometry/ion trap tandem mass spectrometry (FTMS/ITMSMS) that compared normal and degenerative discs showed that in patient samples, 73 proteins in the AF and 54 proteins in the NP exhibited differential expression levels between the two groups (Sarath Babu et al., 2016)