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The tagged proteins are then passed through a column of beads containing covalently attached, immobilized nickel(II) (Ni 2 + .) This His-tag binds tightly to the immobilized metal ions because the side chain of Histidine, imidazole, has a specific binding affinity to metal ions (in this case, nickel II)

Extracts of the medium were incubated at 80 C for at least 15 min before being centrifuged at 15,000 g for 20 min

doi: 10.3389/fimmu.2024.1470283 Received 25 July 2024 Accepted 03 December 2024 Published 19 December 2024 Volume 15 - 2024 Edited by Vijay Kumar, Morehouse School of Medicine, United States Reviewed by Venkateswarlu Kanamarlapudi, Swansea University Medical School, United Kingdom Takashi Yazawa, Asahikawa Medical University, Japan Eytan R Barnea, LLC, United States Updates Copyright 2024 Deng, Chen, Xing, Zhang and Xu