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Add 80 l 10 mM HCl, and lyse the cells by freezing and thawing twice

Furthermore, the OA-associated acidic microenvironments (pH 6.67.2) promote M1 macrophage polarization through altered glucose metabolism, thereby augmenting their pro-inflammatory activity [11]

Patent 5,290,571: Biologically Active Whey Protein Concentrate

The homocysteine-inducible, endoplasmic reticulum (ER) stress-inducible, ubiquitin-like domain member 2 (HERPUD1) in cultured HELA cells facilitate cytoprotective effects against oxidative stress by suppressing the inositol 1,4,5-triphosphate receptor and reducing the transfer of calcium ions from the endoplasmic reticulum to mitochondria [41]