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Figure 2 2 S effectively promotes erectile function ( 2 S is protein persulfidation, through which H 2 S can modify proteins and regulate various signaling pathways ( 2 S also plays a crucial role in facilitating ion transport across the epididymal epithelium ( 2 S can also effectively regulate smooth muscle relaxation in vas deferens ( 2 Hydrogen sulfide in sperm and seminal fluid H 2 S activates several signaling pathways in spermatozoa ( Figure 3 ) which participate in the development of sperm motility, capacitation, and acrosome reaction, such as the activating MAPK pathway involving four central cascades (ERK 1/2, JNK, p38, and ERK5) (63)

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