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doi: 10.3168/jds.2015-10354 10 DangAKPrasadSDeKPalSMukherjeeJSandeepIVet al

The isoelectric point (Ip) of Ala in free form is around 6,02, where is amino group of Ala is protonated (NH 3+) and its carboxyl group (COO-) is deprotonated at the same time, then zwitterion was formed with no net charge