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Cells were collected by centrifugation (15 min, 4,500 g ) and resuspended in the cold lysis buffer containing 50 mM Tris pH 8, 150 mM NaCl, 1 mM PMSF and 5 mM DTT

(2015) Role of Pex21p for piggyback import of Gpd1p and Pnc1p into peroxisomes of Saccharomyces cerevisiae J