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j , Representative IHC images of 4-HNE and MDA staining (left) and corresponding IHC staining scores of MDA (right) in MC38 tumors from C57BL/6J mice with non-adipose or WAT-enriched tumor models after treatment with IKE (scale bar, 50 m)

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Besides substrate availability, GCL is the rate-limiting enzyme in GSH synthesis, which presents two different subunits: the catalytic subunit (GCLC), containing the active site responsible for the bond formation between the amino group of cysteine and the -carboxyl group of glutamic acid, and the modifier subunit (GCLM) that interacts with GCLC, increasing the catalytic efficiency of GCLC [5]