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Subsequently, live cells were stained with 0.5 M H 2 CDFDA (Invitrogen, Netherlands) to assess ROS level or with 1 M MitoSOX Mitochondrial Superoxide Indicators (Invitrogen, Netherlands) to detect mitochondrial ROS level

Yi Wang, [email protected] This article was submitted to Pharmacology of Anti-Cancer Drugs, a section of the journal Frontiers in Pharmacology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

cruzi antigens cross-reacted in vitro with colon and breast cancer cells [74] as well as with intracellular and membranous molecules in lung cancer [76]

Z-stacks with a step size of 0.25 m were acquired and the total cell volume was estimated using a custom CellProfiler pipeline, as has been previously reported ( At least 50 cells were analyzed and the cell culture conditions were kept identical to those used for LC/MS analysis