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Specifically, adding 5-amino-1mq (30 M and above) to C2C12 myoblast cultures (a common in vitro myoblast model) markedly upregulates the expression of myogenic marker genes, including myosin heavy chain (MyHC), actin, and myogenin (MyoG)

[/et_pb_text][et_pb_text background_layout=light text_orientation=center text_line_height=1.9em use_border_color=off border_color=#ffffff border_style=solid custom_margin=-7px||-25px| background_position=top_left background_repeat=repeat background_size=initial module_alignment=center] Compounds have the same affinity even though the binding constants vary by 4 orders of magnitude [/et_pb_text][et_pb_text background_layout=light text_orientation=justified text_font_size=16 text_line_height=1.9em use_border_color=off border_color=#ffffff border_style=solid background_position=top_left background_repeat=repeat background_size=initial] Some compounds have extremely high on rates, meaning there is very little energy needed to form the interaction, but also very high off rates, meaning once the interaction is formed it is relatively weak