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Method of sacrifice After 12 h of hypobaric hypoxia exposure, rats were injected intraperitoneally (IP) with anesthetic agents (Ketamine-Xylazine cocktail with 100:20 ratio)

In a retrospective analysis of 236 patients with esophageal cancer, those who had an MPV of 7.4 fL or less had significantly shorter overall survival than patients with an MPV higher than 7.4 fL

Interestingly, it was reported that GH is not required for normal intrauterine growth, a finding supported by evidence that GH deficiency/insensitivity does not associate with a significant reduced size at birth [31-35]

At the end of the study, final blood samples were taken, and the animals were euthanized by cardiac injection