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Activation of Nrf2/HO-1 signaling: An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress

Haldar K, Mohandas N

doi: 10.1002/cncr.32988 36 KimJ

Western blotting Protein lysates were prepared using 1% sodium dodecyl sulphate (SDS) lysis buffer and resolved by 12.5% SDS-PAGE for 2 hours at 150 mA, followed by dry transfer (i-Blot protein transfer apparatus, Invitrogen, USA) and incubation with primary antibodies