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Description

With the lung infiltration of neutrophils and macrophages triggered by CS, proinflammatory cytokines such as interleukin -1(IL-1) and tumor necrosis factor- (TNF-) induce a cascade of amplification effects of inflammation, and activate nuclear factor- B (NF- B) (Di Stefano et al., 2002)

After the pRBCs are lysed, they will be placed in the PD 10 desalting column

Finally, the membrane was incubated in SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific Pierce Protein Biology) and imaged with ChemiDocTMTouch Imaging system (BioRad)