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For Sirius Red staining, paraffin-embedded adipose tissue sections were deparaffinized and dehydrated with xylene and ethanol

Then, to achieve a hyperthermostability comparable to that of Ph CutA1, we designed ionic mutants in which charged residues were introduced into a hydrophobic mutant ( Ec CutA1_0SH_S11V/E61V) by substitution

Skin prick tests and specific IgE blood tests are generally more likely to be covered compared to alternative methods