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Methylfolate cannot complete the methylation process and breakdown of homocysteine without Methyl B12

2.8 Renal injury biomarkers Kidney Injury Molecule-1 (KIM-1), a highly sensitive early biomarker of proximal tubular damage, was measured in kidney tissue homogenates using ELISA kit (Elabscience Biotechnology Inc., Houston, TX, USA) according to the manufacturers instructions

At 25 C, the conversion of 100 mM GSH was incomplete after 48 h and significantly poorer than that at 37 C, in which almost complete conversion was obtained (Fig