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ROS level regulation and cell metabolism both affect ferroptosis, and both processes are influenced by heat stress: when increased iron uptake or decreased storage causes iron overload, free iron generates ROS via the Fenton reaction, directly triggering ferroptosis-related damage

B.MihaylovaM

studied the ROS content in neutrophils of different age groups and found that the intracellular ROS content increased significantly in people aged 6089 years compared with those aged 2059 years

L929 conditioned media which contains the macrophage growth factor M-CSF, was prepared by culturing L929 cells (ATCC) in complete DMEM (Thermo Fisher Scientific, MT10013CV) supplemented with 10% FBS, and 1% penicillin and streptomycin for 10 days at 37C, 5% CO 2