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The sections were mounted onto 300-mesh copper grids and imaged using a Talos L120C G2 transmission electron microscope (Thermo Fisher Scientific Inc., Waltham, MA, USA) at an acceleration voltage of 120 kV

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After resuspending in PBS, the suspension was extruded through 800 nm and 200 nm porous polycarbonate filters for 11 times to prepare RAW 264.7 cell-membrane nanovesicles (RCM)