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Confocal images were acquired with a Zeiss inverted LSM 789 laser scanning confocal microscope (Zeiss) using a 100x/1.4 DIC Plan-Apochromat oil immersion objective

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In addition, Noxa expression levels strongly increased in liver specimens from NASH patients, and serum ALT levels of NASH patients were positively correlated with expression levels of Noxa in their livers