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Transmission electron microscopy M0 macrophages were stimulated in 6-well plates (5 10 5 cells per well) as described above and then fixed for 30 min with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (Thermo Fisher Scientific)

IC50 values were determined by non-linear least-squares fitting of a 4-parameter dose-response curve to collected data points (Graphpad Prism 7.0

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Ribosome biogenesis acts downstream of Trp prototrophy and inhibits Gcn4 induction Three parallel lines of evidence converged on the hypothesis that the ribosome biogenesis pathway mediates the repressive effect on Gcn4 induction by Trp metabolism: First, under nutrient-rich conditions, ribosomal proteins can repress the translation of GCN4 mRNA as a regulatory mechanism to maintain the Gcn4 protein at a relatively low basal level 51