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However, PTDD without impact loading can be induced effectively in animal models by means of endplate perforation or by annulus fibrosus (AF) laceration

doi: 10.1016/j.brainres.2005.07.005

ROS are a hallmark of oxidative stress and they can damage intracellular proteins, lipids, and DNA, leading to impairment of cellular function and structure, which has been implicated in the development of a variety of diseases including aging, diabetes mellitus, vascular endothelial cell injury, neurodegenerative diseases, and the onset of cellular senescence, strategies to replenish depleted NAD + pools can offer significant improvements of pathologic states (Klimova and Kristian, 2019