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In a study by Zhou et al., erythropoietin pre-treatment significantly upregulated CXCR4 expression, altered surface molecule profiles, reduced adhesion to pulmonary capillaries, and decreased pulmonary retention

While proper loading time is relatively easily to establish and loading temperature is mostly the temperature used for cell culture (for example 37 C and 39 C for human and mouse cells and cell lines, respectively), additional fine-tuning is needed to establish proper loading concentration of the fluorescent dyes to avoid mitochondrial toxicity, non-specific binding to other cellular structures, photodamage and dye aggregate artifacts

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doi: 10.1016/j.redox.2023.102643 76 HenrotinYKurzBAignerT