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Enzyme-capacity constraint flux balance analysis (ecFBA) and flux-sum analysis To investigate metabolic states under various conditions, we utilized the iCHO2291 model, a comprehensive reconstruction of the CHO cell metabolic network 33 , adapted by incorporating experimentally measured uptake and secretion rates as boundary constraints for each condition, with exchange flux bounds for unmeasured or unexpected metabolites set to zero to prevent artificial nutrient uptake, while adjusting the bounds for glucose uptake, lactate secretion, and the biomass reaction to 10% of their measured rates to accommodate potential experimental errors, a range determined to effectively simulate physiological behavior

10.1097/CND.0000000000000077 189 ThomasS.EndersJ.KaiserA.RovenstineL.HeslopL.HauserW.et al (2023)

with the addition of 55mM glucose (B) or carbon equivalent amounts of glycerol (C), hexose phosphates (+10mM glutathione) (D), and glycerol and hexose phosphates (+10mM glutathione) (E)

The ability of these chemicals to induce ferroptosis will also be discussed with special attention paid to their effects in SH-SY5Y human neuroblastoma cells since these cells possess properties of dopaminergic neurons, and, therefore, have a long history of use as an in vitro model in studies on PD (Cheung et al., in vitro model of chemically-induced PD showed that apoptosis is the main mode of cell death induced by PQ (Klintworth et al., 2007