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Cultivation of HT29-MTX cells for 7 days showed a ZO-1 signal in the cytoplasm, while cells cultivated for 28 days showed a ZO-1 signal at the cell-cell connections but not in the cytoplasm

For the discussion below on neurodegenerative diseases, we find it useful to first consider the autophagy pathway defects affecting upstream steps of the pathway followed by the more extensive dysfunctions affecting the digestive lysosomal stage of autophagy, bearing in mind that both are integral stages of the same pathway

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STONE: Oh, incredible